It’s been a while since I updated…
However, I did make quite an interesting observation this morning in lab. Terrific broth cultures, after subjecting them to denaturing conditions with GnHCl, are not pleasant to work with. After spinning down my cultures and getting ready to incubate them for purification, I proceeded to add 20% bleach to the tubes to make it easier to clean. Instead, the solution turned a bright orange upon addition of the bleach and it foams and smells really bad.
I let out a squeal because it was nasty. I can’t shake the smell or feel of it right now. Good thing I was wearing gloves because bleach and bacteria are no good combination ever.
Lesson? Lab safety! You never know what two chemicals can do when placed together.